Monohybrid Cross – Punnett Square Practice Worksheet – PDF & Digital




A four page practice with a short descriptive passage explaining the key vocabulary required in monohybrid crosses. Designed to be implemented either in person, digitally, or hybrid.

Sections include:

  • Vocabulary Matching
  • Practice Identifying Alleles (Dominant, Recessive, Heterozygous, Homozygous)
  • Practice Determining Genotypes and Phenotypes
  • Punnett Square Practice


Please download the preview file for a closer look at the contents of this resource!


Purchase includes a printable PDF file with answer key. On page 2 of this resource you will find a link to a student friendly Google Slide version of this file. You will be able to copy this file and use it with Google Classroom or any other paperless initiative.


Uses for this product:

  • An independent work station in a set of stations
  • Flipped Classroom pre-reading
  • Differentiation – Assign this reading as reteaching for students who have yet to show mastery
  • Homework 
  • Creation of Independent Work Packet for students who are not able to be present for direct instruction.
  • Extension activity for early finishers
  • Use as a square on a Choice Board
  • Interactive Notebooks: Print 2 pages in one and cut apart. Glue mini pages into notebooks with room for annotations on the side
  • Assessment either formative or summative. You can provide the answers to this quiz using a QR code or after students have verified that they’ve completed their work.



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Personal Experience: 

I find that teaching Punnett squares and inheritance is one of the best parts of my job as a middle school science teacher. It’s one time in our curriculum where students are doing instead of memorizing, and I love seeing the joy on their faces when things start to make sense!

I teach monohybrid crosses as a whole group lesson, and then I have a self paced unit set up that students work through on their own. The progression I use is:

    1. Monohybrid Crosses
    2. Codominance, Incomplete Dominance, Blood Types
    3. Sex Linked Traits
    4. Dihybrid Crosses

For each, I have an introductory video (something like Khan Academy). A practice worksheet such as this one, and then a quiz. I usually use Quizizz. In order to move on to the next level, students must achieve 80% on the quiz.

As the unit progresses, I generally find that students group themselves based on ability level and work together to understand the new Punnett squares and to pass the quiz. This frees me up to move around the room. Generally, I spend the majority of my time with the students who are still struggling to grasp monohybrid crosses. Occasionally I sit with the more advanced groups and give them a quick rundown of their new challenge.

At the end of about 2 weeks, I assess the students only up to the level that they were able to master. I’ve found this to be one of my students’ favorite units and one that allows them to feel empowered instead of rushed as they (for the first time in many of their lives) finally have as much time as they need to work through a skill.


NGSS Standards covered by this Punnett square Practice Worksheet:

HS-LS3-2 Make and defend a claim based on evidence that inheritable genetic variations may result from (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors. Emphasis is on using data to support arguments for the way variation occurs. Assessment does not include the phases of meiosis or the biochemical mechanism of specific steps in the process.

HS-LS3-3 Apply concepts of statistics and probability to explain the variation and distribution of expressed traits in a population. Emphasis is on the use of mathematics to describe the probability of traits as it relates to genetic and environmental factors in the expression of traits. Assessment does not include Hardy-Weinberg calculations.


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