Laney Lee

Dihybrid Cross Practice Worksheet



Need some extra practice with dihybrid crosses? This 3 page practice worksheet is just what you’ve been looking for with one page reteaching and two pages of practice.


Who is this resource for?

This resource can be used by classroom teachers, tutors, and parents of students in grades 6-9. It provides a variety of practice covering the mentioned topics.


How Can I Use this Resource?

  • Emergency Sub Plans
  • An independent work station in a set of stations
  • Differentiation – Assign this practice as reteaching for students who have yet to show mastery.
  • Homework
  • Creation of Independent Work Packet for students who are not able to be present for direct instruction.
  • Extension activity for early finishers or for students who show a special interest in the topic
  • Use as a square on a Choice Board
  • Interactive Notebooks: Print 2 pages in one and cut apart. Glue mini pages into notebooks with room for annotations on the side
  • Interactive Notebooks: Print entire PDF as a mini booklet and add to notebooks using these simple instructions.


What’s Included?

Purchase includes a printable PDF file in color with answer key. On page 2 of this resource you will find a link to a student friendly Google Slide version of this file. You will be able to copy this file and use it with Google Classroom or any other paperless initiative.


Please take a look at the preview file to see more of this resource.


More questions?

Email me at


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Personal Experience: 

I find that teaching Punnett squares and inheritance is one of the best parts of my job as a middle school science teacher. It’s one time in our curriculum where students are doing instead of memorizing, and I love seeing the joy on their faces when things start to make sense!

I teach monohybrid crosses as a whole group lesson, and then I have a self paced unit set up that students work through on their own. The progression I use is:

    1. Monohybrid Crosses
    2. Codominance, Incomplete Dominance, Blood Types
    3. Sex Linked Traits
    4. Dihybrid Crosses

For each, I have an introductory video (something like Khan Academy). A practice worksheet such as this one, and then a quiz. I usually use Quizizz. In order to move on to the next level, students must achieve 80% on the quiz.

As the unit progresses, I generally find that students group themselves based on ability level and work together to understand the new Punnett squares and to pass the quiz. This frees me up to move around the room. Generally, I spend the majority of my time with the students who are still struggling to grasp monohybrid crosses. Occasionally I sit with the more advanced groups and give them a quick rundown of their new challenge.

At the end of about 2 weeks, I assess the students only up to the level that they were able to master. I’ve found this to be one of my students’ favorite units and one that allows them to feel empowered instead of rushed as they (for the first time in many of their lives) finally have as much time as they need to work through a skill.


NGSS Standards covered by this  Dihybrid Cross Practice Worksheet:

HS-LS3-2 Make and defend a claim based on evidence that inheritable genetic variations may result from (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors. Emphasis is on using data to support arguments for the way variation occurs. Assessment does not include the phases of meiosis or the biochemical mechanism of specific steps in the process.

HS-LS3-3 Apply concepts of statistics and probability to explain the variation and distribution of expressed traits in a population. Emphasis is on the use of mathematics to describe the probability of traits as it relates to genetic and environmental factors in the expression of traits. Assessment does not include Hardy-Weinberg calculations.


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